Spore testing your autoclave is critical for ensuring that it’s doing its job effectively.
What Happens if The Sterilizer Fails? There are occasions when a sterilizer fails a spore test. These tests are conducted anywhere from weekly to monthly, and a one-time fail is acceptable. Beyond that, action should be taken. From here, we would do a complete maintenance check to look for worn or faulty parts, which might include valves or gaskets. Normal wear and tear on autoclaves is to be expected, and that’s why routine testing and maintenance are so important. These actions can stop an emergency from happening.
Also known as biological indicator tests, spore testing entails introducing non pathogenic spores into your sterilizer to test its function. A spore test looks for indicators that compromise the sterilization process, commonly highly resistant spores.
These spores are first placed onto strips of filter paper, and then, the paper is packed into a glassine paper pouch. This paper is permeable yet not affected by moisture and air.
The glassine pouch is placed in the sterilizer, and the sterilizer is put into operation.
The goal is to monitor sterilization and look at how it kills resistant microorganisms.
The autoclave should effectively do so.
There are numerous factors that are taken into account when it comes to spore testing, including steam and temperature. The FDA, OSHA, and CDC require spore tests at your site at least once per month. In some cases, these tests might be required more frequently. It’s important to be sure that you’re staying on top of spore tests not just for compliance but for the quality that your lab is passing on.